Cyto-id® green detection reagent
WebFeb 1, 2024 · After 24 h of laser irradiation, cells were labeled with CYTO-ID® Green. All the fluorescent images were captured using Cytation5 cell imaging multi-mode reader with objective lens 20×, followed by uniform processing using Cytation5 software (bar = 100 μm). ... To assess autophagy, we used CYTO-ID® Autophagy Detection Kit 2.0 based on a … WebApr 9, 2024 · CYTO-ID ® Green Detection Reagent 2 was stained for 30 min and analyzed by flow cytometry. Compared with untreated cells, the fluorescence was increased, with treatment with RAP + CLQ (Figures 4J,K). The results indicated that autophagy can be induced under stress conditions.
Cyto-id® green detection reagent
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WebCYTO-ID® Autophagy Detection Kit 2.0, Enzo Life Sciences Supplier: Enzo Life Sciences A brighter and more photostable, no-transfection, quantitative assay for monitoring … WebDec 1, 2024 · The Cyto-ID® Green detection reagent which is a vital coloration of autophagic vacuoles can be read with a FITC filter (Excitation ∼480 nm, Emission ∼530), and the Hoechst 33,342 Nuclear Stain can be read with a DAPI filter set (Excitation ∼340, Emission ∼480). This result was examined using iRiS™ Digital Cell Imaging System …
Web1. Product and Company Identification Product Code: 10-4410-xx Product Name: UniCap Phosphoramidite Product Use: For Research Use Only Manufacturer Information Company Name: Glen Research LLC 22825 Davis Drive Sterling, VA 20164 USA Phone Number: 1-703-437-6191 Web site address: www.glenresearch.com Email address: … WebThe Cyto-ID™ Autophagy Detection Kit is a member of the CELLestial® product line, reagents and assay kits comprising fluorescent molecular probes that have been …
WebCYTO-ID Autophagy detection kit (1x200tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer … WebJun 13, 2024 · Inflorescence-based of CYTO-ID® autophagy detection. Primary chondrocytes isolated from the cartilage of WT and GRN −/− mice were treated with DMSO (control) or IL-1β (1 μM) for 24 h, then stained with CYTO-ID® Green Detection Reagent and LysoTracker Red at a concentration of 300 nM was added and incubated at 37°C for …
WebProduct overview. Autophagy Assay Kit ab139484 measures autophagic vacuoles and monitors autophagic flux in live cells using a dye that selectively labels autophagic …
WebThe green dye emits in the green region of the visible light spectrum and is thus not compatible with GFP. b) Flow cytometry application: Make a dye stain solution by diluting 1 μL Green Detection Reagent to 1 mL cell culture medium without Phenol Red Indicator, supplemented with 5% FBS. For flydining priceWebCyto-ID labeling and Flow cytometry: HEK-293 cells were harvested in Accutase solution (Merck Millipore, SF006), rinsed in DPBS and incubated for 30 min at room temperature … greenhouse water collection systemWebThe LYSO-ID ® Green detection kit contains an acidic organelle-selective dye suitable for live-cell staining. Conventional fluorescent stains for acidic organelles, such as Acridine Orange (Prod. No. ENZ-52405), form … greenhouse warehouse hitchinWebThe DYNEX Technologies Automated ELISA System is a cutting edge computer-controlled processing system that achieves full automation of ELISA assays and surrounding … fly dine inWebFeb 14, 2024 · Cyto-ID green detection reagent, consisting of 1 μL Hoechst 33342, 100 μL Cyto-ID reagent per 1 mL cell culture medium supplemented with 10% FBS was used to stained cells. The plate was stored ... fly dining noida menuWebReagents Hypoxia Red Detection Reagent, part of the Cyto-ID® Hypoxia/Oxidative Stress Detection Kit (Catalog No. ENZ-51042) was provided by Enzo Life Sciences (Farmingdale, NY). The reagent is a weakly-fluorescent compound containing a nitro (NO 2) moiety. Due to nitroreductase activity present in hypoxic flydining restaurant himachal pradeshWebEnzo Life Sciences CYTO-ID Autophagy detection kit (1x50tests) A no-transfection, quantitative assay for monitoring autophagy in live cells. CYTO-ID.The probe is a cationic amphiphilic tracer (CAT) dye that rapidly … greenhouse water butt connector kit