Ctg reagent
WebAug 1, 2012 · The assay described makes use of a luciferase-based assay reagent, the Promega CellTiter-Glo (CTG) kit, to measure the conversion of AMP to Ado by 5′-ectonucleotidase. Briefly, the CTG kit measures ATP levels through the luciferase-linked emission of light accompanying catabolism of ATP to AMP and free diphosphate. The …
Ctg reagent
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WebCell-Titer Glo (CTG) reagent (Promega) was dispensed at 4 µl/well using the BioRAPTR FRD Microfluidic Workstation. The plates were incubated in the dark at RT for 10 min prior to detection by the ViewLux plate reader (1 s exposure, 1× binning). To assess if stable expression of lux potentially WebMay 3, 2024 · Caspase-3/7 green detection reagent was used to monitor apoptosis over 12 hours (a,b), and the CTG assay was used to quantify live cells at 24 hours post-thawing (c).
WebMay 26, 2024 · Prepare the CTG reagent following the manufacturer’s instructions. 17. Remove the tissue culture plate from the incubator and leave it 10 min at room … WebMar 30, 2024 · The CTG reagent lyses cellular and mitochondrial membranes and binds to adenosine triphosphate (ATP) released by cells, which produces a luminescence signal. The intensity of the luminescence was considered a proxy measure for the number of live cells in a well. The luminescence intensities were measured using the CLARIOstar plate reader.
WebMay 4, 2024 · The ratio may be varied towards smaller CTG amounts. 14. Store the remaining CTG solution at −20 °C. Reconstituted CTG reagent can be stored at RT for up to 8 h with 10% loss of activity, at 4 °C for 48 h with 5% loss of activity, and at −20 °C for 21 weeks with 3% loss of activity (see the manufacturer’s instructions). The reagent is ... WebMar 6, 2024 · A CTG file is an index file created by Canon digital cameras. It contains information used to organize images stored on a camera's memory card. For example, …
WebFor time course experiments using CTG, cells were incubated with the compounds from 2 to 72 h using a differ - ent plate per time point before adding 25 µL of CTG reagent. For time course experiments using the ImageTOX, cells were incubated with the compounds from 2 to 72 h, and the dye mixture was added 30 min before the first time point.
WebNov 9, 2024 · Equilibrate CTG reagent to room temperature. Following degradation measurement at the last time point of the kinetic analysis, add 100 µL (96-well plate) or 40 µL (384-well plate) of the reagent per well of the plate, and mix on a plate shaker at 500-700 rpm (96-well plate) or a microplate vortex mixer (384-well plate) for 5 min. fish and fire restaurantWebIndustry leaders choose CTG because we accelerate their digital journeys. We ensure digital transformation solutions are delivered with the urgency and outcomes our clients need. … fish and fly forumWebDefine CTG. means a control technique guideline. A CTG is a U.S. EPA guidance document that triggers a responsibilityunder Section 182(b)(2) of the Clean Air Act regarding certain … camworks wire edm proWebFeb 24, 2024 · ChessBase Opening Tree of Games. These CTG files contain chess opening books in a file format created by ChessBase. A chess opening refers to the initial moves … fish and food central gmbhWebPR-G7571. View Documents. View Product Certificates. Promega. G7571. 10 x 10 mL. 1000 x 100 μL assays or 4000 x 25 μL assays. Each for $882.00. Check Availability. cam worrellWebAug 1, 2024 · CTG is a reagent used to determine the number of viable cells in culture based on quantification of the total ATP correlating with cell number. Since ATP and ALP are both presented in cell lysates, it was important to exclude the possibility that one of the agents impacts the luminescent signal of the other. fish and fly magazineWebMar 18, 2024 · Thaw and warm the CTG reagent. 18. Add CTG reagent at 1:3 ratio (50 μL per well of 150 μL culture media) to each well of the 96- well plate containing alginate beads by using a multichannel pipette. 19. Place the plates on a … camworld camera